X-ray Crystallography Core Laboratory

Services

 

Service Price UTHSCSA discount CTRC Discount
Crystal screen (96 conditions), vapor diffusion $27 per plate, $3 extra for each additional protein up to 3 total $24 per plate, $3 extra for each additional protein up to 3 total $21 per plate, $3 extra for each additional protein up to 3 total
Crystal screen (96 conditions), microbatch $27 per plate $24 per plate $21 per plate
Additive Screen $21 per plate, $3 extra for each additional protein up to 3 total $18 per plate, $3 extra for each additional protein up to 3 total $15 per plate, $3 extra for each additional protein up to 3 total
X-ray Diffraction Data Collection $18 per hour $15 per hour $12 per hour
Synchrotron Data Collection $500 per trip
Xenon Derivative $50
Molecular Modeling $50 per hour

 

The X-ray Crystallography Core Laboratory utilizes 14 commercial screens containing 96 crystallization reagents.  In a “full screen,” each commercial screen is set up at two temperatures (22°C and 4°C) with proteins of interest to identify initial crystallization conditions. 

X-ray Core Users will typically provide 700 microliters of a 5-20 mg/ml protein preparation to complete a full screen. It is recommended to avoid phosphate buffer in order to reduce false positives. HEPES, MES, MOPS, PIPES, CHES, Tris, acetate, citrate and bicine are suitable protein buffers for crystallization. NaCl or KCl up to 150 mM concentration is acceptable. Reducing agents DTT, BME, and TCEP are also compatible with most of the crystallization reagents.